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interleukin 7  (R&D Systems)


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    Structured Review

    R&D Systems interleukin 7
    Interleukin 7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+interleukin+7/Recombinant+Human+IL-7+Protein/us12466878-637-0-3
    Average 95 stars, based on 206 article reviews
    interleukin 7 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Hypoxic, glycolytic metabolism is a vulnerability of B-acute lymphoblastic leukemia-initiating cells
    Article Snippet: Human cell lines were obtained from the ATCC. .. Patient derived leukemia cells were cultured on MS5 stromal cells in the presence of 50 ng/mL recombinant human stem cell factor (SCF), 50 ng/mL recombinant human thrombopoietin (TPO), 10 ng/mL recombinant human FLT3 ligand (FLT3L), and 10 ng/mL recombinant human interleukin-7 (IL-7, all from R and D Systems) in Myelocult H5100 (Stem Cell Technologies) supplemented with 1% penicillin/streptomycin ( ) with modifications based on ( ) to facilitate lymphoid cell growth; i.e., without horse serum or hydrocortisone ( ). ..

    Article Title: Single-cell analysis uncovers mechanisms of plasticity in leukemia initiating cells
    Article Snippet: .. Patient derived leukemia cells were cultured on MS5 stromal cells in the presence of 50 ng/ml recombinant human stem cell factor (SCF), 50 ng/ml recombinant human thrombopoietin (TPO), 10 ng/ml recombinant human FLT3 ligand (FLT3L), and 10 ng/ml recombinant human interleukin-7 (IL-7, all from R and D Systems)( ). ..

    Cell Culture:

    Article Title: Hypoxic, glycolytic metabolism is a vulnerability of B-acute lymphoblastic leukemia-initiating cells
    Article Snippet: Human cell lines were obtained from the ATCC. .. Patient derived leukemia cells were cultured on MS5 stromal cells in the presence of 50 ng/mL recombinant human stem cell factor (SCF), 50 ng/mL recombinant human thrombopoietin (TPO), 10 ng/mL recombinant human FLT3 ligand (FLT3L), and 10 ng/mL recombinant human interleukin-7 (IL-7, all from R and D Systems) in Myelocult H5100 (Stem Cell Technologies) supplemented with 1% penicillin/streptomycin ( ) with modifications based on ( ) to facilitate lymphoid cell growth; i.e., without horse serum or hydrocortisone ( ). ..

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..

    Article Title: Effects of hypoxia on antigen presentation and T cell-based immune recognition of HPV16-transformed cells
    Article Snippet: .. PBMCs were thawed and one T cell culture per antigen stimulation was set up with 1–2 × 10 6 cells/well in 24-well plates in T cell medium (RPMI-1640 with 10% FBS, 2 mM L-glutamine, 10 mM HEPES, and 1% P/S) supplemented with 10 ng/ml recombinant human Interleukin 7 (rhIL-7; R&D Systems). .. Each T cell line was stimulated with 10 μg/ml of either one of the 28 HLA-A2-binding HPV16 E6- and E7-derived synthetic peptides, 10 μg/ml HIV-A2 negative control peptide, or 1 μg/ml per peptide of the positive control CEF peptide pool.

    Article Title: Single-cell analysis uncovers mechanisms of plasticity in leukemia initiating cells
    Article Snippet: .. Patient derived leukemia cells were cultured on MS5 stromal cells in the presence of 50 ng/ml recombinant human stem cell factor (SCF), 50 ng/ml recombinant human thrombopoietin (TPO), 10 ng/ml recombinant human FLT3 ligand (FLT3L), and 10 ng/ml recombinant human interleukin-7 (IL-7, all from R and D Systems)( ). ..

    Recombinant:

    Article Title: Hypoxic, glycolytic metabolism is a vulnerability of B-acute lymphoblastic leukemia-initiating cells
    Article Snippet: Human cell lines were obtained from the ATCC. .. Patient derived leukemia cells were cultured on MS5 stromal cells in the presence of 50 ng/mL recombinant human stem cell factor (SCF), 50 ng/mL recombinant human thrombopoietin (TPO), 10 ng/mL recombinant human FLT3 ligand (FLT3L), and 10 ng/mL recombinant human interleukin-7 (IL-7, all from R and D Systems) in Myelocult H5100 (Stem Cell Technologies) supplemented with 1% penicillin/streptomycin ( ) with modifications based on ( ) to facilitate lymphoid cell growth; i.e., without horse serum or hydrocortisone ( ). ..

    Article Title: Hypoxic, glycolytic metabolism is a vulnerability of B-acute lymphoblastic leukemia-initiating cells
    Article Snippet: Recombinant human thrombopoietin , R and D Systems , 288-TP-005. .. Recombinant human interleukin-7 , R and D Systems , 207-IL-005. .. Mitotracker green , Thermo Fisher , CAS: 201860-17-5.

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..

    Article Title: Effects of hypoxia on antigen presentation and T cell-based immune recognition of HPV16-transformed cells
    Article Snippet: .. PBMCs were thawed and one T cell culture per antigen stimulation was set up with 1–2 × 10 6 cells/well in 24-well plates in T cell medium (RPMI-1640 with 10% FBS, 2 mM L-glutamine, 10 mM HEPES, and 1% P/S) supplemented with 10 ng/ml recombinant human Interleukin 7 (rhIL-7; R&D Systems). .. Each T cell line was stimulated with 10 μg/ml of either one of the 28 HLA-A2-binding HPV16 E6- and E7-derived synthetic peptides, 10 μg/ml HIV-A2 negative control peptide, or 1 μg/ml per peptide of the positive control CEF peptide pool.

    Article Title: Single-cell analysis uncovers mechanisms of plasticity in leukemia initiating cells
    Article Snippet: .. Patient derived leukemia cells were cultured on MS5 stromal cells in the presence of 50 ng/ml recombinant human stem cell factor (SCF), 50 ng/ml recombinant human thrombopoietin (TPO), 10 ng/ml recombinant human FLT3 ligand (FLT3L), and 10 ng/ml recombinant human interleukin-7 (IL-7, all from R and D Systems)( ). ..

    Article Title: PEBP 1 suppresses HIV transcription and induces latency by inactivating MAPK / NF ‐κB signaling
    Article Snippet: Naive CD4 + T cells were further purified from peripheral blood mononuclear cells by negative selection according to the manufacturer's instructions (Thermo). .. The Naive CD4 + T cells were maintained in serum‐free medium supplemented with 1% penicillin‐streptomycin and 5 ng/ml recombinant human interleukin‐2 (R&D) and 10 ng/ml recombinant human interleukin‐7 (R&D) at 37°C in 5% CO 2 . ..

    Article Title: FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat
    Article Snippet: Naive CD4 + T cells were further purified from peripheral blood mononuclear cells by negative selection according to the manufacturer's instructions (Thermo Fisher). .. The naive CD4 + T cells were maintained in serum-free medium supplemented with 1% penicillin-streptomycin, 5 ng/ml recombinant human interleukin-2 (R&D Systems), and 10 ng/ml recombinant human interleukin-7 (R&D Systems) at 37°C under 5% CO 2 . ..

    Flow Cytometry:

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..

    Staining:

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..

    Concentration Assay:

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..



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